The fluorescence intensity (indicated by number of positive partitions) for the Supermix no dUTP reaction mix is consistently high, with most values clustering above 3 on the log scale, indicating consistently higher positive partition counts and less variability in fluorescence. In contrast, the RT Supermix reaction mix exhibits a broader range of positive partition values, indicating a lower and more variable fluorescence.
For all pathogens except measles, mean gene copies/µl were higher in concentrations of 105 than 103 regardless of reaction mix. For most pathogens, mean gene copies/µl were greater in the Supermix no dUTP than the One-Step RT Supermix when concentration was held constant.